|
KMID : 0613820080180060782
|
|
Journal of Life Science
2008 Volume.18 No. 6 p.782 ~ p.788
|
|
|
Characterization and Distribution of Glycoconjugates in the Intestines of Sebastes schlegeli, Halichoeres poecilopterus, Bryzoichthys lysimus, and Takifugu pardalis
|
|
Jeong Kil-Nam
|
|
|
Abstract
|
|
|
|
The aim of this work is to show differences in the pattern of glycoconjugate composition in the intestines of four teleostean species (Sebastes schlegeli, Halichoeres poecilopterus, Bryzoichthys lysimus, and Takifugu pardalis). We compared four regions of all species studied. The specimens were processed and stained with nine kinds of biotinylated lectins (DBA, SBA, PNA, BSL-Ⅰ, RCA-Ⅰ, sWGA, UEA-Ⅰ, LCA and Con A). Except for Sebastes schlegeli, no differences between regions were observed. The intestinal epithelium of Halichoeres poecilopterus possessed D-glucose/mannose residues in all regions. β-N-acetyl-D-galactosamine was distinctive along the intestines, although the pattern of diversity was different in Sebastes schlegeli, Bryzoichthys lysimus, and Takifugu pardalis. Additionally, the occurrence of Galactose-β-1,3-N-acetyl-D-galactosamine and α-D-galactose were confirmed in the proximal, middle, and distal intestine of Sebastes schlegeli, while rectal intestine lacked these sugar residues. Along with β-N-acetyl-D-galactosamine, β-N-acetyl-D-glucosamine and D-glucose/mannose were also determined in Bryzoichthys lysimus. Galactose-β-1,3-N-acetyl-D-galactosamine, D-galactose, and D-glucose/mannose were also present in Takifugu pardalis.
|
|
|
KEYWORD
|
|
|
Intestine, glycoconjugate, lectin, teleost, gobletcell
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|